What time of day are mice most active? 8 PM - 12 AM 22% 12 AM - 4 AM 22% There is no signficant difference 55% Get Free Quotes Today! Question: What time of day are mice most active? Top Answer (55% of 9 votes): There is no signficant difference. Answer: There is no signficant difference Explanation: I find them active at all times. Patrick's Pest Control Answer: 12 AM - 4 AM Explanation: Mice are most active at night but can be seen in the day as well. Fall Guys LLC Answer: 8 PM - 12 AM Explanation: They are usually out in the late evenings all the way to mid morning. The Wildlife Specialist LLC Get Free Quotes Today! Find Local Pros Free Rodent Control Services Cost Estimates Related Questions Can house mice transmit any diseases to humans?Top answer: Yes Do deer mice transmit any diseases to humans?Top answer: Yes Does a rat infestation pose a significant threat to my health?Top answer: Yes Are ultrasonic devices an effective rodent repellent?Top answer: No, never When is the best time of year to get rid of chipmunks?Top answer: There is no significant difference.